The purpose of the present work was to describe α amylase inhibition and antioxidant ability of three different seaweeds (Velaniopsis pachynema, Chnoospora minima, Gracilaria fergusonii) from Kanyakumari coast. These seaweeds are sequentially subjected to extraction using Methanol, Diethyl ether and Hexane. α Amylase inhibitors are used to control over hyperglycemia in type 2 diabetes mellitus also these inhibitors from natural sources can minimize the toxicity and side effects instead of using synthetic inhibitors to control hyperglycemia. The alpha amylase inhibition assay was carried out in only methanol extracts of seaweeds. The highest inhibition was recorded in Chnoospora minima (45 %, concentration of 50 µg/ml). Obviously, the antioxidant activity was determined by the scavenging assays. In the assay, high total phenol content was observed in methanol extract of Chnoospora minima (9.412±0.001 mg GAE/g) least in hexane extract of Velaniopsis pachynema (1.021±0.020 mg GAE/g). In total antioxidant activity the maximum level was observed in methanolic extract of Chnoospora minima (6.475±0.01 mg AAE /g) less in Velaniopsis pachynema (1.003±0.013 mg AAE /g). In FRAP assay the highest value recorded in Chnoospora minima (6.160±0.03%) and least value shown in Velaniopsis pachynema (0.924±0.05%). Among the seaweed extracts, the scavenging ability against hydrogen peroxide was highly shown in methanol extract of Chnoospora minima (7.003±0.03%) and fewer in Velaniopsis pachynema (0.962±0.01). The methanol extract of Chnoospora minima (4.082±0.05%) shown highest value against DPPH Scavenging assay and low value showed in Velaniopsis pachynema (0.831±0.01%) and the Deoxyribose scavenging assay methanol extract of Chnoospora minima shown high ability (6.275±0.01%) and less in (0.563±0.06%). Because of the polar nature the methanol extract shown high activity in all the assays and considering, hexane (non polar) shows least ability in the entire scavenging assay.
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